Submit Manuscript  

Article Details


Genotyping and Drug Susceptibility Patterns of M Tuberculosis Isolated from HIV Seropositive Patients in Tehran Iran

Author(s):

Seifu Gizaw Feyisa*, Mehrnaz Rasoolinejad, Maliheh Hasan-nejad, Siroos Amini, Gholamreza Hamzelou, Jalil Kardan-Yamchi, Hossein Kazemian, Morteza Karami-Zarandi and Mohammad Mehdi Feizabadi*   Pages 1 - 9 ( 9 )

Abstract:


Aim: to investigate the prevalence and drug-resistance M. tuberculosis isolated from HIV seropositive individuals in Tehran, Iran.

Background: Human immunodeficiency virus (HIV) is one of the most important risk factors for developing active tuberculosis (TB).

Objective: To determine the rate of transmission and drug resistant M. tuberculosis (MTB) strains isolated from HIV seropositive patients in Tehran province, Iran.

Methods: This study consecutively enrolled 217 TB/HIV coinfected patients from April 2018 to August 2019 at Emam Khomeini referral hospital and 5 other health centers in Tehran province. The isolates were genotyped using 15 loci Mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTR). Minimum inhibitory concentration (MIC) was determined for 6 drugs. In addition, mutations were assessed in rpoB, katG, inhA, and ahpC genes using Reverse Blot Hybridization Assay System.

Results: A 20 (9.2%) patients were culture-positive for M. tuberculosis and typed by MIRU-VNTR, 13 (65%) strains formed 5 clusters, but 6 (30%) isolates had a unique pattern. The total Hunter–Gaston discrimination index (HGDI) for all 15 loci was 0.846, and the cluster size was 2 to 4 patients. The estimated proportion of recent transmission was 45%. The mutation was identified in 1 isolate, lost inhAW1 and mutation in MT1 loci, which was resistant to isoniazid (INH). Moreover, 1 (5%) and 3 (15%) isolates were resistant to INH and ethambutol (EMB), respectively, of which 1 was resistant to INH and EMB.

Conclusion: The transmission rate of TB in HIV patients was relatively high; however, the prevalence of drug-resistant strains and TB infection in females was insignificant in this study (p < 0.05); none of the isolates was MDR strains.

Keywords:

HIV, M. tuberculosis, Mutation, Cluster, MIRU-VNTR, Minimum Inhibitory Concentration, Reverse Blot Hybridization

Affiliation:

Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Department of Infectious Diseases, School of Medicine, Tehran University of Medical Sciences, Tehran, Department of Infectious Diseases, School of Medicine, Tehran University of Medical Sciences, Tehran, Referral Tuberculosis Laboratory, Tehran University of Medical Sciences, Tehran, Referral Tuberculosis Laboratory, Tehran University of Medical Sciences, Tehran, Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Ilam University of Medical Sciences, Clinical Microbiology Research Center, Tehran University of Medical sciences, Tehran, Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran



Read Full-Text article