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MicroRNAs Profiling in HIV, HCV, and HIV/HCV Co-infected Patients

Author(s):

Mohsen Moghoofei, Sohrab Najafipour, Shayan Mostafaei , Ahmad Tavakoli , Farah Bokharaei-Salim , Saied Ghorbani, Davod Javanmard, Hadi Ghaffari and Seyed Hamidreza Monavari*   Pages 1 - 8 ( 8 )

Abstract:


Background: Human immunodeficiency virus (HIV) an hepatitis C virus (HCV) infections are important public health issues.

Objective: This study aimed to assess the association between microRNAs expression level and immunological and viral markers in HIV, HCV, and HIV/HCV coinfected patients.

Method: The expression level of miR-29, miR-149, miR-199, miR-let7, miR-223, miR-155, miR-122, and miR-150 was evaluated in 20 HIV, 20 HCV, 20 coinfected patients and 20 healthy controls using real-time PCR assay. HIV and HCV viral loads were measured by real-time PCR and also, CD4+ T-lymphocyte count was measured by the PIMA CD4 analyzer.

Result: The miRNA expression pattern in each mentioned group showed significantly different expression profiles, but some miRNA species were shared between the groups. MiR-122 and miR-155 were up-regulated while miR-29 and miR-223 were down-regulated in three patients groups compared to healthy controls. A significant positive correlation was observed between the expression of miR-122 and HIV/HCV loads. But, miR-29 and let-7 were negatively correlated with HIV load, and miR149 and let-7 were negatively correlated with HCV load. Also, miR-155 was positively correlated with HCV load. MiR-122 and miR-199 were negatively while others were positively correlated with CD4+ T cell count.

Conclusion: These miRNAs are probably involved in the clinical progression and pathogenesis of HIV and HCV infections. Therefore, determining and manipulating of these miRNAs can lead to opening a new gate to control of these important infections.

Keywords:

HIV, HCV, Coinfection, microRNA, PBMC, AIDS

Affiliation:

Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Department of Microbiology, Faculty of Medicine, Fasa University of Medical Sciences, Fasa, Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Department of Bacteriology and Virology, School of Medicine, Semnan University of Medical Sciences, Semnan, Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran



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