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The Effect of Antiretroviral Therapy on IL-6, IL-1β, TNF-α, IFN-γ Levels and their Relationship with HIV-RNA and CD4+ T Cells in HIV Patients

Author(s):

Gülay Okay*, Meliha Meric Koc, Eray Metin Guler, Ayşegül Yabaci, Abdürrahim Kocyigit and Yasemin Akkoyunlu   Pages 1 - 8 ( 8 )

Abstract:


Background: Serum cytokine levels over the course of HIV infection usually increase with immunosuppression and decrease after antiretroviral treatment (ART).

Objectives: Compare cytokine levels between HIV-infected patients (HIP) and controls and investigate the relationship between CD4+T cell count, HIV-RNA levels, and cytokine levels.

Methods: The study subjects comprised ART-naive HIP (n=30) with no comorbidities and age-and sex-matched healthy controls. We measured levels of IL-6, IL-1β, TNF-α, and IFN-γ in serum samples of HIP at the beginning and at month 6 of ART and in controls.

Results: The mean age of the study subjects was 38.7 ±10.3 years, with men making up 86.7% of the study subjects (n=26). IL-6, IL-1β, and TNF-α levels were significantly higher in both ART-naive (p<0.001, p=0.002, p=0.001) and ARTexperienced HIP (p<0.001) than controls. The IFN-γ level was lower in both ART-naive and ART-experienced HIP compared to controls (p=0.082 and p=0.002). There was a positive correlation between the CD4+T cell count and serum concentration of IFN-γ(r=0.320, p<0.05). While the serum IFN-γ concentration showed a negative correlation with the HIV-RNA level(r=-0.412, p<0.001), the serum IL-1β, IL-6, and TNF-α concentrations showed a positive correlation with the HIVRNA level (r=0.349, p<0.001; r:0.54, p<0.001; r:0.438, p<0.00).

Conclusions: Althugh serum concentrations of IL-6, IL-1β and TNF-a showed significant decrease after ART, they were still significantly higher than the controls. IFN-γ responded differently to ART compared to the other cytokines, indicating that it may play a distinct and important role in the pathogenesis of HIV infection.

Keywords:

IL-6, IL-1β, TNF-α, IFN-γ, HIV infection, cytokines, antiretroviral treatment

Affiliation:

Bezmialem Vakif University, Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Istanbul, Bezmialem Vakif University, Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Istanbul, Bezmialem Vakif University, Faculty of Medicine, Department of Medical Biochemistry, Istanbul, Bezmialem Vakif University, Department of Biostatistics , Bezmialem Vakif University, Faculty of Medicine, Department of Medical Biochemistry, Istanbul, Bezmialem Vakif University, Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Istanbul



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