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Assessment of Key Elements in the Innate Immunity System Among Patients with HIV, HCV, and Coinfections of HIV/HCV

Author(s):

Maryam Moradi, Alireza Tabibzadeh, Davod Javanmard, Saied Ghorbani, Farah Bokharaei-Salim, Hosein Keivani, Mohammad Khazeni and Seyed Hamid Reza Monavari*   Pages 1 - 7 ( 7 )

Abstract:


Background:Coinfection of Hepatitis C virus (HCV) with human immunodeficiency virus (HIV) has a higher risk of mortality than HCV or HIV monoinfection. HCV and HIV infections are specified by systemic inflammation but the inflammation process in HCV/HIV co-infection are much complicated and are not well characterized.

Objective: The aim of this study was to analyze the expression of TLR-3, TLR-7, IL-10, IFN-1 (IFN-α, IFN-β), and TNF-α in HIV, HCV and HIV/HCV co-infected patients. 1

Methods: Forty-five patients including HIV group (n=15), HCV group (n=15), HIV/HCV coinfection group (n=15) and healthy control group (n=15) participated. Peripheral blood mononuclear cells (PBMCs) were obtained. PBMC-RNA, HCV and HIV RNA were extracted from all subjects and cDNA were synthesis. The viral load analyzed by reverse transcription-quantitative PCR (RT‐qPCR), as well, the expression levels of IFN-α, IFN-β, TLR-3, TLR-7, TNF, and IL-10 mRNA were quantified in PBMCs.

Results: The levels of IFN-I, IL-10, and TNF-α were overexpressed in all patients’ groups (p<0.05), TLR-7 was up regulated in all groups, but this up regulation was not statistically significant (p>0.05). TLR-3 showed decrease in all patient groups (p<0.05). The statistical analysis demonstrated that TLR-3 has a negative correlation with HIV load, whereas other genes positively correlated with HIV load. In addition, TLR-3, TNF-α, and IFN-I were negatively correlated with HCV load, whereas TLR-7 and IL10 s were positively correlated with HCV load.

Conclusion: Our results showed a significant relationship between the expression level of innate immunity genes and inflammation in HCV, HIV, and HIV/HCV coinfected patients.

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Affiliation:

Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Department of Virology, School of Medicine, Tehran University of Medical Sciences, Tehran, Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran



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