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High Seroprevalence of Human Herpesvirus 8 Infection in HIV-positive Homosexual Men in Jiangsu Province, China

[ Vol. 15 , Issue. 4 ]

Author(s):

Jiayan Li, Jinyun Song, Guolei Tan, Jianfang Wang, Zhenxian Zhou and Xuping Wu   Pages 273 - 278 ( 6 )

Abstract:


Background: HIV-infected homosexual men are more frequently diagnosed with Kaposi's sarcoma. With the increase of HIV-infected homosexual men in China, we urgently need to know the KS-related human herpesvirus (KSHV/HHV-8) seroprevalence in this population. To investigate HHV-8 prevalence among HIV-positive homosexual men, we recruited 183 patients naive of antiretroviral therapy (ART) whose blood samples presented with HIV-antibody positive as confirmed by western blot.

Methods: HIV viral load was tested using Cobas TaqMan HIV-1 test Version 2.0, and CD4 T cell counts were tested using a Flow cytometry instrument. All HIV-positive blood samples were collected and screened for KSHV. Immunofluorescence (IFA) test was conducted for HHV-8-Specific antibodies (anti-LANA) in the plasma. HHV-8 DNA in whole blood cells of IFA-positive subjects was quantified with Real-time polymerase chain reaction (RT-PCR).

Results: All samples showed HIV RNA positive. CD4+ T cell count was 23% cases (42/183) which showed ≤200 cells/μL, 51.3% cases (94/183) showed 201-500 cells/μL and 25.7% cases (47/183) showed ≥501 cells/μL. Immunofluorescence (IFA) test demonstrated an HHV-8 prevalence of 50.8% (93/183), among which 20.4% of the cases (19/93) were HHV-8 DNA positive. HHV-8 infection showed no difference among different age groups (p=0.96). Similarly, HHV-8 infection exhibited no significant difference among different HIV viral load groups (p=0.08). However, HHV-8 infection among different CD4+ T cell count showed significant difference (P=0.0004).

Conclusion: This study showed a high seroprevalence of human herpesvirus 8 infection in HIVpositive homosexual men.

Keywords:

HHV-8/KSHV, seroprevalence, homosexual men, HIV-positive, CD4, IFA, Real-time polymerase chain reaction.

Affiliation:

The Second Hospital of Nanjing Affiliated to Medical School of Southeast University, Nanjing, 210003, The Second Hospital of Nanjing Affiliated to Medical School of Southeast University, Nanjing, 210003, The Second Hospital of Nanjing Affiliated to Medical School of Southeast University, Nanjing, 210003, The Second Hospital of Nanjing Affiliated to Medical School of Southeast University, Nanjing, 210003, Nanjing Kindmed Clinical Laboratory,Co., Ltd., Nanjing, Jiangsu 210042, The Second Hospital of Nanjing Affiliated to Medical School of Southeast University, No.1-1, Zhongfu Road, Nanjing, Jiangsu 210003

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