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HLA-C Single Nucleotide Polymorphism Associated with Increased Viral Load Level in HIV-1 Infected Individuals from Northeast Brazil

[ Vol. 15 , Issue. 4 ]

Author(s):

Ronaldo Celerino da Silva, Ronald Rodrigues Moura, Antonio Victor Campos Coelho, Luiz Cláudio Arraes, Lucas André Cavalcanti Brandão, Sergio Crovella and Rafael Lima Guimarães*   Pages 266 - 272 ( 7 )

Abstract:


Background: Genetic variations in Human leukocyte antigen C (HLA-C), Zinc ribbon domain containing 1 (ZNRD1) and its antisense RNA (ZNRD1-AS1) genes are known to influence the HIV-1 replication and disease progression.

Objective and Method: We evaluated the distribution of HLA-C (rs10484554, rs9264942) and ZNRD1 (rs8321) and ZNRD1-AS1 (rs3869068), single nucleotide polymorphisms (SNPs) in 266 HIV-1-infected and 223 unexposed-uninfected individuals from Northeast Brazil and their relation to HIV-1 infection, CD4 T cells count and viral load pre-treatment.

Results: HLA-C SNPs were in Linkage Disequilibrium (D’=0.84), constituting four possible haplotypes. Our results showed that HLA-C, ZNRD1 and ZNRD1-AS1 SNPs as well as HLA-C haplotypes frequencies were not significantly different between HIV-1-infected and unexposed-uninfected individuals. In addition, we analyzed HLA-C and ZNRD-1 and ZNRD1-AS1 SNPs considering CD4+ T cell counts and viral load before the antiretroviral treatment. Individuals carrying HLA-C rs9264942 TT genotype showed a significant increased level of HIV-1 viral load pre-treatment, in comparison with individuals carrying the CC genotype (p-value = 0.0092). Finally, we stratified our findings according to CCR5Δ32 allele presence along with the studied SNPs: no statistically significant influence over viral load pre-treatment has been found.

Conclusion: The association between HLA-C rs9264942 SNP and viral load prior treatment in an admixed population from North East Brazil was in agreement with findings from previous studies obtained on different ethnic groups; however more studies should be conducted in order to clarify how HLA-C impair the HIV-1 replication

Keywords:

HLA-C, ZNRD1, ZNRD1-AS1, SNPs, HIV-1 infection, viral load.

Affiliation:

Department of Genetics, Federal University of Pernambuco (UFPE), Recife, Department of Genetics, Federal University of Pernambuco (UFPE), Recife, Department of Genetics, Federal University of Pernambuco (UFPE), Recife, Laboratory of Immunopathology Keixo Asami (LIKA), Federal University of Pernambuco (UFPE), Recife, Laboratory of Immunopathology Keixo Asami (LIKA), Federal University of Pernambuco (UFPE), Recife, Department of Genetics, Federal University of Pernambuco (UFPE), Recife, Department of Genetics, Federal University of Pernambuco (UFPE), Av. Prof. Moraes Rego, 1235 - Cidade Universitária, Zip code 50670-901, Recife

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